000			03583nam a2200469Ii 4500
001			9780429458187
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005			20220724194343.0
006			m o d
007			cr
008			181112t20182019fluab ob 001 0 eng d
020			_a9780429458187 _q(e-book : PDF)
035			_a(OCoLC)1049801226
040			_aFlBoTFG _cFlBoTFG _erda
041	1		_aeng
050		4	_aQP551
072		7	_aMED _x008000 _2bisacsh
072		7	_aPS _2bicscc
082	0	4	_a 612/.01575
100	1		_aBonner, Philip, _eauthor.
245	1	0	_aProtein Purification / _cby Philip Bonner.
250			_aSecond edition.
264		1	_aBoca Raton, FL : _bTaylor & Francis, _c[2018].
264		4	_c©2019.
300			_a1 online resource (287 pages) : _b125 illustrations, text file, PDF.
336			_atext _2rdacontent
337			_acomputer _2rdamedia
338			_aonline resource _2rdacarrier
490	1		_aTHE BASICS (Garland Science)
504			_aIncludes bibliographical references and index.
505	0	0	_t1. Water, pH and buffers. 2. Protein structure and properties. 3. Chromatography and the strategy of protein purification. 4. Groundwork. 5. Non-affinity absorption techniques used to purify proteins. 6. Affinity-based procedures used to purify proteins. 7. Non-absorption techniques for purifying proteins. 8. Methods for monitoring the purity of protein solutions. Appendix 1: The laws of thermodynamics and the Gibbs free energy equation. Appendix 2: Properties of amino acids. Appendix 3: The common units used in biology. Appendix 4: Chromatographic runs. Appendix 5: Determining the concentration of a compound in a solution in parts per million (ppm) Appendix 6: Theanswers totheexercises in the various chapters. Appendix 7: An alphabetical list of chromatography, electrophoresis, filtration equipment and laboratory suppliers. Appendix 8: Buffer tables to prepare at a required pH value.
520	3		_aThis second edition of Protein Purification provides a guide to the major chromatographic techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. The new edition of the book has been organized to encourage incremental learning about the topic, starting with the properties of water, progressing through the characteristics of amino acids and proteins which relate to the purification process. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and their applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.Key Features* Guide to the major techniques used in protein purification* Includes flowcharts to help the reader slect the best purification strategy* Contains step-by-step protocols that guide the reader through each technique and its use* Includes exercises and solutions
530			_aAlso available in print format.
650		0	_aProteins _xPurification.
650		0	_aProteins _xisolation & purification.
655		0	_aElectronic books.
710	2		_aTaylor and Francis.
776	0	8	_iPrint version: _z9781138312470
830		0	_aBASICS (Garland Science).
856	4	0	_uhttps://www.taylorfrancis.com/books/9780429458187 _zClick here to view.
942			_2lcc _cEBK
999			_c16636 _d16636